مرکز تحقیقات بیولوژی کاربردی تکوین جانوری

Studies on stem cells have created new possibilities for osseous defects therapies. This expremental study
focused on the synergic effects of saffron extract and vitamin D3 on osteogenesis differentiation in bone
marrow mesenchyme stem cells (BMSCs) of wistar rat. BMSCs were isolated from rat’s femur and treated
with different doses of saffron extract, vitamin D3 and combination saffron extract and vitamin D3.
Extract cytotoxicity was measured using MTT assay. The alkaline phosphatase (ALP) enzyme activity,
Alizarin red staining and RT-PCR were performed to evaluate the differentiation progress. MTT assay
indicated saffron extract, vitamin D3 and a combination used of those factor decreases cell viability. ALP
activity showed that saffron extract, Vitamin D3 and application of two factors together increase ALP
activity. Alizarin red staining showed vitamin D3, saffron and synergic used of them affects cell
mineralization. RT-PCR result demonstrates in 14 days the group that treated with vitamin D3 and saffron
Together express osteocalcin gene.