مرکز تحقیقات بیولوژی کاربردی تکوین جانوری

Background: Holothuroids (sea cucumbers) are members of the phylum echinodermata, which
produce saponins. Saponins exhibit a wide spectrum of pharmacological and biological activities. In
this study, we isolated the crude saponins from the body wall of the dominant Iranian species of sea
cucumber, Holothuria leucospilota (H. leucospilota). The purpose of this study was to confirm the
presence of saponins in the Persian Gulf H. leucospilota and study the hemolytic and cytotoxic
activities of these compounds.
Methods: The body wall of sea cucumber was dried and powdered and the crude saponins were
isolated using various solvents. The crude saponins were further purified by column
chromatography using HP-20 resin. The foam test, Thin Layer Chromatography (TLC), hemolytic
assay, and Fourier Transform Infrared Spectroscopy (FTIR) confirmed the presence of saponins.
Cytotoxicity was analyzed using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide
(MTT) assay on A549 cells, a human lung cancer cell line.
Results: The foam test, hemolytic assay, and TLC supported the presence of saponin compounds in
the 80% ethanol fraction of H. leucospilota. The infrared (IR) spectrum of the extract showed
hydroxyl (-OH), alkyl (C-H), ether (C-O) and ester (–C=O) absorption characteristic of
teriterpenoid saponins. The C-O-C absorption indicated glycoside linkages to the sapogenins. The
crude saponin extracted from sea cucumber was cytotoxic to A549 cells.
Conclusion: The 80% ethanol fraction of saponin isolated from H. leucospilota exhibited hemolytic
activity and offers promise as an anti-cancer candidate.