مرکز تحقیقات بیولوژی کاربردی تکوین جانوری

In the present study, the effect of red (Gracillaria
corticata), green (Ulva fasciata) and brown (Sargassum ilicifolium) seaweeds alcoholic extract, against five important
human cancer cell lines (MCF-7, MDA-MB-231, HeLa,
HepG2, and HT-29) proliferation, apoptosis and cell cycle
arrest were evaluated. The reducing activity and total polyphenol content were also investigated. MTT assay was used
for cytotoxicity test. Morphological alterations were examined using phase contrast, fluorescent and electron microscopy. All the extracts were antiproliferative against all the
cancer cell lines, dose-dependently, with G. corticata
methanol extract (GCME) having the greatest inhibition
activity against MCF-7 cell line. The percentage of apoptosis
increased from 18 to 78 %. The cell cycle analysis also
showed that GCME can induce apoptosis which confirm by
TEM. Algal extract reducing activities were as follows: G.
corticata[S. ilicifolium [U. fasciata. The GCME is a
good source of potential complementary and alternative
functional food for prevention and treatment of cancer.